{"id":205,"date":"2018-03-14T22:18:03","date_gmt":"2018-03-14T21:18:03","guid":{"rendered":"http:\/\/projects.nib.si\/aureoprotect\/?page_id=205"},"modified":"2018-03-20T15:03:28","modified_gmt":"2018-03-20T14:03:28","slug":"project-plan","status":"publish","type":"page","link":"https:\/\/projects.nib.si\/aureoprotect\/project-plan\/","title":{"rendered":"Project plan"},"content":{"rendered":"

The search for more efficient antagonistic strains against phytopathogens to support both the economic feasibility and practical efficiency of biocontrol methods is an ongoing process. The proposed project aims to identify and characterize new strains of A. pullulans<\/em> or even new species of the genus Aureobasidium<\/em> (for instance A. subglaciale<\/em>) with improved tolerance to stress that can be used in biocontrol of selected plant pathogens.<\/p>\n

This will be achieved by exploiting the rich resources of the EX Culture Collection, which contains numerous strains of Aureobasidium<\/em> spp. from various environments (moderate climate plants from local environment, plants from salterns in the Mediterranean, from Artic glacial ice etc).<\/p>\n

This collection of Aureobasidium<\/em> spp. strains will be screened for antagonistic effects on selected plant pathogens (fungal and bacterial) in vitro <\/em>and in situ. <\/em>We will identify strains that are more stress tolerant (to low and high temperatures, drought, salts and UV irradiation) than commercially available strains presently used for biocontrol.<\/p>\n

The genomes of the selected strains with promising biocontrol potential will be re-sequenced by next-generation sequencing (NGS). The generated data will be subjected to the appropriate analysis to uncover traits relevant to the biocontrol. The basis of the mechanism for bacterial or fungal antagonism will be addressed by screening their enzymatic activity, production of siderophores or other compounds such as volatile organic compounds.<\/p>\n

Finally we will develop the real-time polymerase chain reaction (PCR)-based method for molecular detection of the strain of Aureobasidium<\/em> with the best potential for use in the biocontrol. The method will discriminate between the introduced and indigenous Aureobasidium<\/em> strains.<\/p>\n

Based on gained results, we will evaluate the possibility of using new strains of Aureobasidium<\/em> spp. for biocontrol against certain plant pathogens before and after harvest, either individually or in combinations of various strains.<\/p>\n

The project consists of 4 work packages (WPs), three of which include scientific research, whereas the forth WP comprises the project management and knowledge dissemination. Each WP comprises up to five tasks (T), which are described below.<\/strong><\/p>\n

WP1: Screening of a collection of Aureobasidium<\/em> spp. strains for antagonism <\/strong><\/p>\n

Screening of antagonism of Aureobasidium<\/em> spp. strains (A. pullulans<\/em> and A. subglaciale<\/em>) from the EX Culture Collection (Mycosmo) and commercially used biocontrol strains against the selected fungal and bacterial phytopathogens in vitro<\/em> (on nutrient media) and in situ<\/em> (on fruits or detached blossoms) under relevant stressful conditions.<\/p>\n

The antagonism to the following plant pathogens will be tested on three postharvest fungal pathogens, namely Penicillium expansum<\/em> (blue mold), Botrytis cinerea<\/em> (grey mold), Colleotrichum acutatum<\/em> (bitter rot) and one preharvest bacterial pathogen Erwinia amylovora, <\/em>the causative agent of fire blight.<\/p>\n

T1.1 The first screening or in vitro<\/em> antagonism to selected plant pathogens<\/p>\n

The collection of Aureobasidium<\/em> strains will be evaluated for their interactions with P. expansum<\/em>, B. cinerea<\/em>, and C. acutatum<\/em> in culture according to previously employed procedure (Mari et al.<\/em>, 2012b). The strains with distinguishable antagonism towards tested pathogens will be further evaluated for their biocontrol potential under stressful conditions. Finally, the list of Aureobasidium<\/em> spp. strains will be prepared and used in the second screening.<\/p>\n

T1.2 The second screening or in situ<\/em> antagonism to selected plant pathogens<\/p>\n

In the second screening the selected strains with in vitro<\/em> antagonistic potential will be used for the evaluation of in situ <\/em>antagonistic activity; for postharvest fungal pathogens on apples and for bacteria E. amylovora<\/em> on detached apple blossoms.<\/p>\n

Milestone 1: The list of Aureobasidium<\/em> spp. strains with the best antagonistic activity in vitro<\/em> and in situ<\/em><\/u><\/p>\n

 <\/p>\n

WP2: Phenotypic and genotypic characterisation of selected Aureobasidium<\/em> spp. strains<\/strong><\/p>\n

WP2A Phenotypic characterisation of selected Aureobasidium<\/em> spp.<\/p>\n

T2.1 Extracellular enzymes production<\/p>\n

T2.2 Iron depletion or siderophore production<\/p>\n

T2.3 Production of inhibitory volatile organic compounds<\/p>\n

Milestone 2: <\/u>Phenotypic characterization of the selected <\/u><\/em>Aureobasidium<\/u><\/em> spp. will be completed.<\/u><\/em><\/p>\n

WP2B Genotypic characterisation of selected Aureobasidium<\/em> spp.<\/p>\n

T2.4 Genome sequencing and assembly<\/p>\n

T2.5 Genome analysis for traits related to the biocontrol<\/p>\n

Milestone 3: Completed genotypic characterization of the selected <\/u><\/em>Aureobasidium<\/u><\/em> spp..<\/u><\/em><\/p>\n

\u00a0<\/strong><\/p>\n

WP3: Development of the method for the detection and quantification of the specific Aureobasidium<\/em> spp.<\/strong><\/p>\n

T3.1 Development of the method for the detection and quantification of specific Aureobasidium<\/em> spp.<\/p>\n

Milestone 4: Method for detection and quantification of specific <\/u><\/em>Aureobasidium<\/u><\/em> spp. developed.<\/u><\/em><\/p>\n

\u00a0<\/strong><\/p>\n

DS4: Project management and dissemination of knowledge<\/strong><\/p>\n

T4.1 Supervision of the work, evaluation of results and delivery of milestones.<\/p>\n

T4.2 Dissemination of knowledge.<\/p>\n

Milestone 5: The data obtained during the project are published.<\/u><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

The search for more efficient antagonistic strains against phytopathogens to support both the economic feasibility and practical efficiency of biocontrol methods is an ongoing process. The proposed project aims to identify and characterize new strains of A. pullulans or even new species of the genus Aureobasidium (for instance A. subglaciale) with improved tolerance to stress […]<\/p>\n","protected":false},"author":19,"featured_media":0,"parent":0,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-205","page","type-page","status-publish","hentry"],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/P9J1Tb-3j","jetpack-related-posts":[{"id":14,"url":"https:\/\/projects.nib.si\/aureoprotect\/summary\/","url_meta":{"origin":205,"position":0},"title":"Summary","author":"ablejec","date":"05\/04\/2016","format":false,"excerpt":"The increasing concern about the usage of antimicrobials and other chemicals in food production due to their negative environmental impacts and health risks led to the search for the alternative means of controlling plant diseases. One of the most promising approaches is the use of microbial antagonists that prevent the\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":40,"url":"https:\/\/projects.nib.si\/aureoprotect\/","url_meta":{"origin":205,"position":1},"title":"AureoProtect: short description","author":"ablejec","date":"05\/04\/2016","format":false,"excerpt":"The goal of the project is the application of new strains of A. pullulans or A. subglaciale with improved antagonistic activity against the most problematic plant pathogens and increased tolerance to environmental stress (e.g. low temperatures, UV radiation, low nutrient availability and desiccation). To reach this goal is of utmost\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"arrs-logo-en","src":"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/arrs-logo-en.gif?resize=350%2C200","width":350,"height":200},"classes":[]},{"id":99,"url":"https:\/\/projects.nib.si\/aureoprotect\/links\/","url_meta":{"origin":205,"position":2},"title":"Links","author":"ablejec","date":"12\/04\/2016","format":false,"excerpt":"Links to other variants of project web pages: SICRIS \/ ARRS NIB \/ Projekti","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":60,"url":"https:\/\/projects.nib.si\/aureoprotect\/partners\/","url_meta":{"origin":205,"position":3},"title":"Partners","author":"ablejec","date":"06\/04\/2016","format":false,"excerpt":"\u00a0 National Institute of Biology, Ljubljana, Slovenia University of Ljubljana, Biotechnical Faculty, Department of Biology Ex Culture Collection, Infrastructural centre Mycosmo, MRIC UL","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"NIB","src":"https:\/\/i0.wp.com\/projects.nib.si\/aureoprotect\/wp-content\/uploads\/sites\/15\/2016\/04\/NIBlogo.png?resize=350%2C200","width":350,"height":200},"classes":[]},{"id":30,"url":"https:\/\/projects.nib.si\/aureoprotect\/references\/","url_meta":{"origin":205,"position":4},"title":"Related publications","author":"ablejec","date":"05\/04\/2016","format":false,"excerpt":"ARTICLES AND OTHER COMPONENT PARTS Published scientific conference contribution abstract (invited lecture) 1. GOSTIN\u010cAR, Cene, TURK, Martina, ZAJC, Janja, ZALAR, Polona, GUNDE-CIMERMAN, Nina. Fungi in the salterns - from halotolerance to halophily. V: MaFNaP 2017, 2nd International Conference of Marine Fungal Natural Products (MaFNaP), 27-29 June 2017, Kiel, Germany. [S.\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"_links":{"self":[{"href":"https:\/\/projects.nib.si\/aureoprotect\/wp-json\/wp\/v2\/pages\/205","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/projects.nib.si\/aureoprotect\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/projects.nib.si\/aureoprotect\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/aureoprotect\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/aureoprotect\/wp-json\/wp\/v2\/comments?post=205"}],"version-history":[{"count":2,"href":"https:\/\/projects.nib.si\/aureoprotect\/wp-json\/wp\/v2\/pages\/205\/revisions"}],"predecessor-version":[{"id":208,"href":"https:\/\/projects.nib.si\/aureoprotect\/wp-json\/wp\/v2\/pages\/205\/revisions\/208"}],"wp:attachment":[{"href":"https:\/\/projects.nib.si\/aureoprotect\/wp-json\/wp\/v2\/media?parent=205"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}