{"id":493,"date":"2023-07-18T15:13:32","date_gmt":"2023-07-18T14:13:32","guid":{"rendered":"http:\/\/projects.nib.si\/diavir\/?p=493"},"modified":"2023-07-18T15:21:13","modified_gmt":"2023-07-18T14:21:13","slug":"succesful-sequencing","status":"publish","type":"post","link":"https:\/\/projects.nib.si\/diavir\/2023\/07\/18\/succesful-sequencing\/","title":{"rendered":"Virus infection experiment"},"content":{"rendered":"<p>In May 2023 we carried out a infection progression experiment together with dr. Tinkara Tinta and master student on ERASMUS internship Anna\u00eblle Boeffel.<\/p>\n<p>The experiment consisted of 3 control and 3 virus treatment batches of 1 liter. We measured DOC, nutrients, diatom, bacterial abundance, viral abundance, bacterial 16S tags, and diatom transcriptomes. The data is currently being analyzed and a qPCR assay for the determination of viral loads in the experiment is being tested. Here&#8217;s a pic of how the cultures looked after the experiment:<\/p>\n<p><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" data-attachment-id=\"496\" data-permalink=\"https:\/\/projects.nib.si\/diavir\/2023\/07\/18\/succesful-sequencing\/img_20230512_133526\/\" data-orig-file=\"https:\/\/i0.wp.com\/projects.nib.si\/diavir\/wp-content\/uploads\/sites\/51\/2023\/07\/IMG_20230512_133526.jpg?fit=4000%2C3000&amp;ssl=1\" data-orig-size=\"4000,3000\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;1.8&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;FP3&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;1683898526&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;3.56&quot;,&quot;iso&quot;:&quot;100&quot;,&quot;shutter_speed&quot;:&quot;0.004739336492891&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"IMG_20230512_133526\" data-image-description=\"\" data-image-caption=\"\" data-medium-file=\"https:\/\/i0.wp.com\/projects.nib.si\/diavir\/wp-content\/uploads\/sites\/51\/2023\/07\/IMG_20230512_133526.jpg?fit=300%2C225&amp;ssl=1\" data-large-file=\"https:\/\/i0.wp.com\/projects.nib.si\/diavir\/wp-content\/uploads\/sites\/51\/2023\/07\/IMG_20230512_133526.jpg?fit=840%2C630&amp;ssl=1\" class=\"alignnone size-full wp-image-496\" src=\"https:\/\/i0.wp.com\/projects.nib.si\/diavir\/wp-content\/uploads\/sites\/51\/2023\/07\/IMG_20230512_133526.jpg?resize=840%2C630\" alt=\"IMG_20230512_133526\" width=\"840\" height=\"630\" srcset=\"https:\/\/i0.wp.com\/projects.nib.si\/diavir\/wp-content\/uploads\/sites\/51\/2023\/07\/IMG_20230512_133526.jpg?w=4000&amp;ssl=1 4000w, https:\/\/i0.wp.com\/projects.nib.si\/diavir\/wp-content\/uploads\/sites\/51\/2023\/07\/IMG_20230512_133526.jpg?resize=300%2C225&amp;ssl=1 300w, https:\/\/i0.wp.com\/projects.nib.si\/diavir\/wp-content\/uploads\/sites\/51\/2023\/07\/IMG_20230512_133526.jpg?resize=768%2C576&amp;ssl=1 768w, https:\/\/i0.wp.com\/projects.nib.si\/diavir\/wp-content\/uploads\/sites\/51\/2023\/07\/IMG_20230512_133526.jpg?resize=1024%2C768&amp;ssl=1 1024w, https:\/\/i0.wp.com\/projects.nib.si\/diavir\/wp-content\/uploads\/sites\/51\/2023\/07\/IMG_20230512_133526.jpg?w=1680 1680w, https:\/\/i0.wp.com\/projects.nib.si\/diavir\/wp-content\/uploads\/sites\/51\/2023\/07\/IMG_20230512_133526.jpg?w=2520 2520w\" sizes=\"auto, (max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 1362px) 62vw, 840px\" \/><\/p>\n<p>The first three are virus treatments and the last three are controls. There is clearly much more &#8220;brown&#8221; color in the controls, whereas the viral treatments look diluted and discolored. Stay tuned!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>In May 2023 we carried out a infection progression experiment together with dr. Tinkara Tinta and master student on ERASMUS internship Anna\u00eblle Boeffel. The experiment consisted of 3 control and 3 virus treatment batches of 1 liter. We measured DOC, nutrients, diatom, bacterial abundance, viral abundance, bacterial 16S tags, and diatom transcriptomes. The data is &hellip; <a href=\"https:\/\/projects.nib.si\/diavir\/2023\/07\/18\/succesful-sequencing\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Virus infection experiment&#8221;<\/span><\/a><\/p>\n","protected":false},"author":48,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[1],"tags":[],"class_list":["post-493","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p7OqMV-7X","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/projects.nib.si\/diavir\/wp-json\/wp\/v2\/posts\/493","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/projects.nib.si\/diavir\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/projects.nib.si\/diavir\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/diavir\/wp-json\/wp\/v2\/users\/48"}],"replies":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/diavir\/wp-json\/wp\/v2\/comments?post=493"}],"version-history":[{"count":3,"href":"https:\/\/projects.nib.si\/diavir\/wp-json\/wp\/v2\/posts\/493\/revisions"}],"predecessor-version":[{"id":497,"href":"https:\/\/projects.nib.si\/diavir\/wp-json\/wp\/v2\/posts\/493\/revisions\/497"}],"wp:attachment":[{"href":"https:\/\/projects.nib.si\/diavir\/wp-json\/wp\/v2\/media?parent=493"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/projects.nib.si\/diavir\/wp-json\/wp\/v2\/categories?post=493"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/projects.nib.si\/diavir\/wp-json\/wp\/v2\/tags?post=493"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}