{"id":277,"date":"2018-03-07T16:06:51","date_gmt":"2018-03-07T15:06:51","guid":{"rendered":"http:\/\/projects.nib.si\/gliostemcell\/?page_id=277"},"modified":"2022-08-02T11:18:03","modified_gmt":"2022-08-02T10:18:03","slug":"project-phases-and-realisation","status":"publish","type":"page","link":"https:\/\/projects.nib.si\/gliostemcell\/project-phases-and-realisation\/","title":{"rendered":"Project phases and realisation"},"content":{"rendered":"
\"Shema_BB_postdoc

Experimental overview of the research project (work packages and tasks).<\/p><\/div>\n

 <\/p>\n

Work package (WP) 1: Exploring the presence of mesenchymal stem cells (MSCs) and their association with glioblastoma (GBM) cell and GBM stem cells (GSC) therapeutic sensitivity in patient-derived organotypic multicellular spheroids (OMSs) and patient clinical data <\/strong><\/p>\n

Task 1.1: Detection of MSCs in OMSs<\/p>\n

Task 1.2: Evaluation of cytotoxic effects of irradiation and TMZ treatment on GBM cells and GSCs in OMSs<\/p>\n

Task 1.3: Correlation of the amount of MSCs in OMSs with cell responses to treatment and clinical data<\/p>\n

Realization<\/strong>:\u00a0complete<\/p>\n

WP 2: Evaluation of the effects of MSCs on GSC therapeutic sensitivity in co-cultures in vitro <\/em><\/strong><\/p>\n

Task 2.1: Evaluation of GSC proliferation, apoptosis and cell cycle progression<\/p>\n

Task 2.2: Evaluation of GSC self-renewal, stemness and differentiation<\/p>\n

Task 2.3: Evaluation of DNA damage responses in GSCs<\/p>\n

Task 2.4: Evaluation of GSC migration and invasion<\/p>\n

Task 2.5: Analysis of cytokine release in co-culture media<\/p>\n

Task 2.6: Evaluation of inhibition of candidate cytokine signaling on GSC treatment sensitivity in vitro<\/em><\/p>\n

Realization:<\/strong>\u00a0complete<\/p>\n

WP 3: Studying the effects of MSCs on GSC therapeutic sensitivity in zebrafish embryo brain in vivo <\/em><\/strong><\/p>\n

Task 3.1: Evaluation of cell proliferation and invasion<\/p>\n

Task 3.2: Evaluation of GSC stemness, differentiation and expression of GSC niche proteins in mixed tumors<\/p>\n

Task 3.3: Evaluation of inhibition of candidate cytokine signaling on GSC treatment sensitivity in vivo<\/em><\/p>\n

Realization:<\/strong>\u00a0complete<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

  Work package (WP) 1: Exploring the presence of mesenchymal stem cells (MSCs) and their association with glioblastoma (GBM) cell and GBM stem cells (GSC) therapeutic sensitivity in patient-derived organotypic multicellular spheroids (OMSs) and patient clinical data Task 1.1: Detection of MSCs in OMSs Task 1.2: Evaluation of cytotoxic effects of irradiation and TMZ treatment […]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-277","page","type-page","status-publish","hentry"],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/P7OqMV-4t","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/projects.nib.si\/gliostemcell\/wp-json\/wp\/v2\/pages\/277","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/projects.nib.si\/gliostemcell\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/projects.nib.si\/gliostemcell\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/gliostemcell\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/gliostemcell\/wp-json\/wp\/v2\/comments?post=277"}],"version-history":[{"count":11,"href":"https:\/\/projects.nib.si\/gliostemcell\/wp-json\/wp\/v2\/pages\/277\/revisions"}],"predecessor-version":[{"id":377,"href":"https:\/\/projects.nib.si\/gliostemcell\/wp-json\/wp\/v2\/pages\/277\/revisions\/377"}],"wp:attachment":[{"href":"https:\/\/projects.nib.si\/gliostemcell\/wp-json\/wp\/v2\/media?parent=277"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}