Ongoing:

WP1: Establishing GB organoids (GBOs) from patient tumours to capture the entire glioblastoma microenvironment.

Preparing for:

WP2: In situ sequencing on glioblastoma organoid sections to visualize which cell types and genes are affecting glioblastoma microenvironment upon standard treatment.

The ISS methodology is set up in the lab at NIB. 

ISS workflow scheme.

Ongoing:

WP3: Chemokine-receptor interactions within the glioblastoma microenvironment with proximity ligation assay in response to treatment.

We are preparing first samples.