In NanoBreak project, we investigate the impact of nanoplastics originating from the environment, generated from degraded plastics collected in European coastal areas.

These materials better represent what our bodies are actually exposed to in daily life, including both the particles themselves and the additives and chemical substances released during degradation.

WP1: Production and physico-chemical characterization of eNPs and its leachates (M1-M20)

Task 1.1: Selection of sampling locations and sampling (M1-M12)

Task 1.2: Classification and characterisation of eMPs (M3-M14)

Task 1.3: eNPs production from eMPS (M6-M14)

Task 1.4: Leachates production from eMPs and characterisation (M9-M20)

WP2: Innovative experimental models to support 3R principles (M6-M56)

Task 2.1: Advancing human liver organoids from hiPSCs: A platform for eNP toxicity studies (M6-M56)

Task 2.2: Zebrafish embryos to unveil the impact of eNPs (M12-M56)

Task 2.3: Models for induced liver injury in h-LO and zebrafish embryos to study resilience to eNPs exposure (M24-M56)

WP3:  High-Resolution Analysis of eNPs Interactions, Transformations, and Fate in Biological Systems (M16-M56)

Task 3.1:  Integrated Raman spectroscopy and NanoSIMS characterization of eNPs’ molecular interactions in biological systems (M16-M46)

Task 3.2: Investigating biomolecular interactions and toxicological impacts of eNPs (M26-M56)

WP4: A spatial multi-omics approach to investigate the impact of eNPs in Human Liver Organoids and Zebrafish Embryos; (M26-M58)

Task 4.1: Unravelling metabolic shifts: Spatial metabolomics of eNPs effects in h-LO and zebrafish embryos (M26-M50)

Task 4.2:  Decoding transcriptomic changes: Spatial transcriptomics of eNPs effects in h-LO and zebrafish embryos (M30-M54)

Task 4.3:  Mapping epigenetic changes: Spatial epigenomics of eNPs effects in h-LO and zebrafish embryos (M36-M58)

Task 4.4: Development of a pipeline for integrating metabolomic, transcriptomic, and epigenomic data to uncover comprehensive responses to eNPs (M26-M58)

WP5: Management and coordination; (M1-M60)

Task 5.1: Administrative and Financial Management (M1-M60)

Task 5.2: Scientific and Technical Management (M1-M60)

WP6: Dissemination, Exploitation and Communication; (M1-M60)

Task 6.1: Dissemination, Exploitation and Communication (DE&C) Strategy (M1-M60)  

Task 6.2: Data Management plan (M1-M60)

Task 6.3: IPR Management (M6-M60)

Task 6.4: Monitoring and Evaluation of DE&C Activities (M6-M60)