{"id":40,"date":"2016-04-05T22:31:14","date_gmt":"2016-04-05T22:31:14","guid":{"rendered":"http:\/\/projects.nib.si\/rnainval\/?page_id=40"},"modified":"2020-02-14T20:55:32","modified_gmt":"2020-02-14T19:55:32","slug":"short-description","status":"publish","type":"page","link":"https:\/\/projects.nib.si\/rnainval\/","title":{"rendered":"Short description"},"content":{"rendered":"<div id=\"attachment_116\" style=\"width: 202px\" class=\"wp-caption alignright\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-116\" data-attachment-id=\"116\" data-permalink=\"https:\/\/projects.nib.si\/rnainval\/short-description\/cpb\/\" data-orig-file=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/cpb.jpg?fit=1296%2C1926&amp;ssl=1\" data-orig-size=\"1296,1926\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;NIKON D80&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;1281097317&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;320&quot;,&quot;shutter_speed&quot;:&quot;0.01&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;1&quot;}\" data-image-title=\"CPB larvae\" data-image-description=\"\" data-image-caption=\"&lt;p&gt;CPB larvae on potato leaf&lt;\/p&gt;\n\" data-medium-file=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/cpb.jpg?fit=202%2C300&amp;ssl=1\" data-large-file=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/cpb.jpg?fit=689%2C1024&amp;ssl=1\" class=\" wp-image-116\" src=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/cpb.jpg?resize=192%2C285\" alt=\"CPB\" width=\"192\" height=\"285\" srcset=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/cpb.jpg?resize=202%2C300&amp;ssl=1 202w, https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/cpb.jpg?resize=768%2C1141&amp;ssl=1 768w, https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/cpb.jpg?resize=689%2C1024&amp;ssl=1 689w, https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/cpb.jpg?w=1296&amp;ssl=1 1296w\" sizes=\"auto, (max-width: 192px) 100vw, 192px\" \/><p id=\"caption-attachment-116\" class=\"wp-caption-text\">CPB larvae feeding on potato leaf (Photo: Marko Petek)<\/p><\/div>\n<p>This project\u2019s main objective is to identify novel <strong>Colorado potato beetle<\/strong> (CPB) gene targets for the development of specific <a href=\"https:\/\/en.wikipedia.org\/wiki\/Gene_silencing_pesticide\" target=\"_blank\">RNAi insecticides<\/a> and further validate prospective targets using transcriptomics. The work\u00a0includes gene selection, establishment of double-stranded RNA (dsRNA) production methodology and the evaluation of dsRNAs\u2019 insecticidal potential in CPB\u00a0feeding trials.<\/p>\n<p>The\u00a0global changes induced by the RNAi insecticide on the CPB gut will be examined using <strong><a href=\"https:\/\/en.wikipedia.org\/wiki\/RNA-Seq\" target=\"_blank\">RNA-Seq<\/a><\/strong>. To explore how the\u00a0RNAi insecticide changes the bacterial community structure and expression of genes in CPB guts, the larval gut contents will be sequenced using NextGeneration Sequencing (NGS) to obtain<strong> <a href=\"https:\/\/en.wikipedia.org\/wiki\/Metagenomics\" target=\"_blank\">metagenomics\/metatranscriptomic<\/a> profiles<\/strong>.<\/p>\n<p><a href=\"http:\/\/projects.nib.si\/rnainval\/summary\/\">More&#8230;<\/a><\/p>\n<p>This project is financed by the\u00a0<a href=\"https:\/\/www.arrs.gov.si\/en\/index.asp\" target=\"_blank\">Slovenian Research Agency (ARRS)<\/a>.<br \/>\nProject ID:\u00a0Z4-7068<br \/>\nDuration:\u00a01.11.2014 &#8211; 31.10.2017<\/p>\n<p><a href=\"https:\/\/www.arrs.si\/\" rel=\"attachment wp-att-239\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" data-attachment-id=\"239\" data-permalink=\"https:\/\/projects.nib.si\/rnainval\/short-description\/arrs-logo-en\/\" data-orig-file=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/arrs-logo-en.gif?fit=322%2C92&amp;ssl=1\" data-orig-size=\"322,92\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"arrs-logo-en\" data-image-description=\"\" data-image-caption=\"\" data-medium-file=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/arrs-logo-en.gif?fit=300%2C86&amp;ssl=1\" data-large-file=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/arrs-logo-en.gif?fit=322%2C92&amp;ssl=1\" class=\"alignleft size-full wp-image-239\" src=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/04\/arrs-logo-en.gif?resize=322%2C92\" alt=\"arrs-logo-en\" width=\"322\" height=\"92\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>This project\u2019s main objective is to identify novel Colorado potato beetle (CPB) gene targets for the development of specific RNAi insecticides and further validate prospective targets using transcriptomics. The work\u00a0includes gene selection, establishment of double-stranded RNA (dsRNA) production methodology and the evaluation of dsRNAs\u2019 insecticidal potential in CPB\u00a0feeding trials. The\u00a0global changes induced by the RNAi [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":138,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-40","page","type-page","status-publish","has-post-thumbnail","hentry"],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/P7OqMV-E","jetpack-related-posts":[],"jetpack_likes_enabled":true,"_links":{"self":[{"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/pages\/40","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/comments?post=40"}],"version-history":[{"count":18,"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/pages\/40\/revisions"}],"predecessor-version":[{"id":309,"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/pages\/40\/revisions\/309"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/media\/138"}],"wp:attachment":[{"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/media?parent=40"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}