{"id":174,"date":"2016-05-23T15:14:22","date_gmt":"2016-05-23T14:14:22","guid":{"rendered":"http:\/\/projects.nib.si\/rnainval\/?p=174"},"modified":"2017-03-06T12:27:49","modified_gmt":"2017-03-06T11:27:49","slug":"cpb-target-genes-selected","status":"publish","type":"post","link":"https:\/\/projects.nib.si\/rnainval\/2016\/05\/23\/cpb-target-genes-selected\/","title":{"rendered":"dsRNAs for CPB target genes synthesised"},"content":{"rendered":"<p>For each target gene a dsRNA was designed (the <a href=\"http:\/\/www.dkfz.de\/signaling\/e-rnai3\/idseq.php\" target=\"_blank\">E-RNAi web service<\/a>\u00a0was used to optimise primer design)\u00a0and synthesised <em>in vitro<\/em> by <a href=\"http:\/\/www.agrorna.com\/index.html\" target=\"_blank\">agroRNA<\/a>. In addition, the positive and negative control dsRNA for the forthcoming feeding experiments were synthesised.<\/p>\n<div id=\"attachment_175\" style=\"width: 418px\" class=\"wp-caption aligncenter\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-175\" data-attachment-id=\"175\" data-permalink=\"https:\/\/projects.nib.si\/rnainval\/2016\/05\/23\/cpb-target-genes-selected\/dsrna_ciscena_50x_2_cropped\/\" data-orig-file=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/05\/dsrna_ciscena_50x_2_cropped.jpg?fit=408%2C340&amp;ssl=1\" data-orig-size=\"408,340\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"dsRNAs\" data-image-description=\"\" data-image-caption=\"&lt;p&gt;in vitro synthesised dsRNAs on a agarose gel&lt;\/p&gt;\n\" data-medium-file=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/05\/dsrna_ciscena_50x_2_cropped.jpg?fit=300%2C250&amp;ssl=1\" data-large-file=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/05\/dsrna_ciscena_50x_2_cropped.jpg?fit=408%2C340&amp;ssl=1\" class=\"size-full wp-image-175\" src=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/05\/dsrna_ciscena_50x_2_cropped.jpg?resize=408%2C340\" alt=\"in vitro synthesised dsRNAs on a agarose gel\" width=\"408\" height=\"340\" srcset=\"https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/05\/dsrna_ciscena_50x_2_cropped.jpg?w=408&amp;ssl=1 408w, https:\/\/i0.wp.com\/projects.nib.si\/rnainval\/wp-content\/uploads\/sites\/4\/2016\/05\/dsrna_ciscena_50x_2_cropped.jpg?resize=300%2C250&amp;ssl=1 300w\" sizes=\"auto, (max-width: 408px) 100vw, 408px\" \/><p id=\"caption-attachment-175\" class=\"wp-caption-text\">agarose gel of<em> in vitro<\/em> synthesised dsRNAs (50x diluted)<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"<p>For each target gene a dsRNA was designed (the E-RNAi web service\u00a0was used to optimise primer design)\u00a0and synthesised in vitro by agroRNA. In addition, the positive and negative control dsRNA for the forthcoming feeding experiments were synthesised.<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"status","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[5,6],"tags":[9,8,7,10],"class_list":["post-174","post","type-post","status-publish","format-status","hentry","category-research-progress","category-wet-lab","tag-dsrna-design","tag-dsrna-synthesis","tag-electrophoresis","tag-experimental-controls","post_format-post-format-status"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p7OqMV-2O","jetpack-related-posts":[],"jetpack_likes_enabled":true,"_links":{"self":[{"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/posts\/174","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/comments?post=174"}],"version-history":[{"count":6,"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/posts\/174\/revisions"}],"predecessor-version":[{"id":180,"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/posts\/174\/revisions\/180"}],"wp:attachment":[{"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/media?parent=174"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/categories?post=174"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/projects.nib.si\/rnainval\/wp-json\/wp\/v2\/tags?post=174"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}