{"id":215,"date":"2018-03-19T13:27:51","date_gmt":"2018-03-19T12:27:51","guid":{"rendered":"http:\/\/projects.nib.si\/spiny\/?page_id=215"},"modified":"2018-03-19T13:27:51","modified_gmt":"2018-03-19T12:27:51","slug":"phases-and-realization","status":"publish","type":"page","link":"https:\/\/projects.nib.si\/spiny\/phases-and-realization\/","title":{"rendered":"Phases and Realization"},"content":{"rendered":"<p><strong>WP1 <\/strong><strong>Cloning, expression and mutagenesis of PVY proteins and selected potato proteins (IJS, NIB)<\/strong><\/p>\n<p>-Task 1.1 Selection of virus and potato plant proteins (NIB). (Months 1-6) <strong>Done<\/strong><\/p>\n<p>-Task 1.2 High throughput expression and purification (IJS). Months (3-18) <strong>Done<\/strong><\/p>\n<p>-Task 1.3 Mutagenesis of proteins involved in interactions identified in WP3 (IJS, NIB). Months (18-30) <strong>(Ongoing)<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>WP2 <\/strong><strong>PVY coat protein and virus like particles VLPs: production, assembly and structure (KI, NIB)<\/strong><\/p>\n<p>-Task 2.1. Production of PVY VLPs. Purification and characterization.(KI, NIB) Months (1-24)<\/p>\n<p><em>-Sub task 2.1.1: Expression conditions (1-8).<strong>Done<\/strong><\/em><\/p>\n<p><em>-Sub task 2.1.2: Specific molecular interactions involved in the assembly (8-16). <strong>Done<\/strong><br \/>\n<\/em><\/p>\n<p><em>-Sub task 2.1.3. Intrinsic coat protein properties (16-24) <strong>Done<\/strong><br \/>\n<\/em><\/p>\n<p>&nbsp;<\/p>\n<p>-Task 2.2. CP subunit structure. (KI) Months (12-36) <strong>Ongoing<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>WP3 <\/strong><strong>Searching, identification and evaluation of new interaction partners within the PVY-potato system (NIB, KI).<\/strong><\/p>\n<p>-Task 3.1. Searching for interaction partners in potato plant extracts (NIB, KI) (1-20)<\/p>\n<p><em>-Sub task 3.1.1 Preparation of plant extracts (months 1-6) <strong>Done<\/strong><\/em><\/p>\n<p><em> -Sub task 3.1.2 SPR experiments (months 6-14) <strong>Done<\/strong><\/em><\/p>\n<p><em>-Sub task 3.1.3 Identification of potential interaction partners (months 14-20) <strong>Ongoing<\/strong><br \/>\n<\/em><\/p>\n<p>&nbsp;<\/p>\n<p>-Task 3.2. Searching for interactions among virus particles or VLPs, and expressed virus and plant proteins (NIB, KI).<\/p>\n<p><em>-Subtask 3.2.1 Interaction screening (months 6-24) <strong>Done<\/strong><br \/>\n<\/em><\/p>\n<p><em>-Subtask 3.2.2 Detailed analysis of confirmed interactions (months 22-36) <strong>Done<\/strong><br \/>\n<\/em><\/p>\n<p>&nbsp;<\/p>\n<p>-Task 3.3. Confirmation of identified interactions with other approaches (28-36) <strong>Ongoing<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>WP4 <\/strong><strong>Mathematical modelling of PVY host interaction (NIB) (Months 28-36) Ongoing<br \/>\n<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>WP1 Cloning, expression and mutagenesis of PVY proteins and selected potato proteins (IJS, NIB) -Task 1.1 Selection of virus and potato plant proteins (NIB). (Months 1-6) Done -Task 1.2 High throughput expression and purification (IJS). Months (3-18) Done -Task 1.3 Mutagenesis of proteins involved in interactions identified in WP3 (IJS, NIB). Months (18-30) (Ongoing) &nbsp; [&hellip;]<\/p>\n","protected":false},"author":12,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-215","page","type-page","status-publish","hentry"],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/P7Rl1h-3t","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/projects.nib.si\/spiny\/wp-json\/wp\/v2\/pages\/215","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/projects.nib.si\/spiny\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/projects.nib.si\/spiny\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/spiny\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/projects.nib.si\/spiny\/wp-json\/wp\/v2\/comments?post=215"}],"version-history":[{"count":1,"href":"https:\/\/projects.nib.si\/spiny\/wp-json\/wp\/v2\/pages\/215\/revisions"}],"predecessor-version":[{"id":217,"href":"https:\/\/projects.nib.si\/spiny\/wp-json\/wp\/v2\/pages\/215\/revisions\/217"}],"wp:attachment":[{"href":"https:\/\/projects.nib.si\/spiny\/wp-json\/wp\/v2\/media?parent=215"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}