Publications resulting from the project:

PETEK, Marko, GRUDEN, Kristina. Selection and validation of novel targets for RNAi-based control of Colorado potato beetle. In: Creating the plant RNAi research network: Cost Action CA15223: book of abstracts, 1st Cost iPlanta Conference, February 15-17, 2017, University Sapienza and CNR Headquarters, Rome, Italy. [S. l.: s. n., 2017]. [COBISS.SI-ID 4249679]


Previous publications of the project’s working group related to this project:

Petek M, Rotter A, Kogovšek P et al. (2014) Potato virus Y infection hinders potato defence response and renders plants more vulnerable to Colorado potato beetle attack. Molecular Ecology, 23, 5378–91.

Šmid I, Gruden K, Buh Gašparič M et al. (2013). Inhibition of the growth of Colorado potato beetle larvae by macrocypins, protease inhibitors from the parasol mushroom.
Journal of Agricultural Food Chemisty, 61, 12499-509.

Petek M, Turnšek N, Buh Gašparič M et al. (2012) A complex of genes involved in adaptation of Leptinotarsa decemlineata larvae to induced potato defense. Archives of Insect Biochemistry and Physiology, 79, 153–81.

Gruden K, Kuipers AG, Gunčar G et al. (2004) Molecular basis of Colorado potato beetle adaptation to potato plant defence at the level of digestive cysteine proteinases. Insect Biochemistry and Molecular Biology34, 365-75.


A list of publications related to RNAi research in insects from other authors:

Casacuberta JM, Devos Y, du Jardin P et al. (2015) Biotechnological uses of RNAi in plants: risk assessment considerations. Trends in biotechnology, 33, 145–7.

Fu K-Y, Guo W-C, Ahmat T, Li G-Q (2015) Knockdown of a nutrient amino acid transporter gene LdNAT1 reduces free neutral amino acid contents and impairs Leptinotarsa decemlineata pupation. Scientific Reports, 5, 18124.

Fu K-Y, Li Q, Zhou L-T et al. (2015) Knockdown of juvenile hormone acid methyl transferase severely affects the performance of Leptinotarsa decemlineata (Say) larvae and adults. Pest management science.

Guo W-C, Fu K-Y, Yang S, Li X-X, Li G-Q (2015) Instar-dependent systemic RNA interference response in Leptinotarsa decemlineata larvae. Pesticide Biochemistry and Physiology, 28–33.

Guo Z, Kang S, Zhu X et al. (2015) The novel ABC transporter ABCH1 is a potential target for RNAi-based insect pest control and resistance management. Scientific Reports, 5, 13728.

Ivashuta S, Zhang Y, Wiggins BE et al. (2015) Environmental RNAi in herbivorous insects. RNA, 21, 840–850.

Li H, Khajuria C, Rangasamy M et al. (2015) Long dsRNA but not siRNA initiates RNAi in western corn rootworm larvae and adults. Journal of Applied Entomology, 139, 432–445.

San Miguel K, Scott JG (2015) The next generation of insecticides: dsRNA is stable as a foliar-applied insecticide. Pest Management Science.

Tan J, Levine SL, Bachman PM et al. (2015) No impact of DvSnf7 RNA on honey bee (Apis mellifera L.) adults and larvae in dietary feeding tests. Environmental Toxicology and Chemistry, 35, 287–294.

Ulrich J, Dao VA, Majumdar U et al. (2015) Large scale RNAi screen in Tribolium reveals novel target genes for pest control and the proteasome as prime target. BMC Genomics, 16, 674.

Zhang H, Li H, Guan R, Miao X (2015) Lepidopteran insect species-specific, broad-spectrum, and systemic RNA interference by spraying dsRNA on larvae. Entomologia Experimentalis et Applicata.

Zhang J, Khan SA, Hasse C et al. (2015) Full crop protection from an insect pest by expression of long double-stranded RNAs in plastids. Science, 347, 991–4.

Zhu T-T, Meng Q-W, Guo W-C, Li G-Q (2015) RNA interference suppression of the receptor tyrosine kinase Torso gene impaired pupation and adult emergence in Leptinotarsa decemlineata. Journal of Insect Physiology, 83, 53–64.

Dubelman S, Fischer J, Zapata F et al. (2014) Environmental fate of double-stranded RNA in agricultural soils. PLoS ONE, 9, 1–7.

Fu K-Y, Guo W-C, Lü F, Liu X, Li G (2014) Response of the vacuolar ATPase subunit E to RNA interference and four chemical pesticides in Leptinotarsa decemlineata (Say). Pesticide biochemistry and physiology, 114, 16–23.

Palli SR (2014) RNA interference in Colorado potato beetle: steps toward development of dsRNA as a commercial insecticide. Current Opinion in Insect Science, 6, 1–8.

Ramon M, Devos Y, Lanzoni A et al. (2014) RNAi-based GM plants: food for thought for risk assessors. Plant Biotechnology Journal, 12, 1271–3.

Wang J, Wu M, Wang B, Han Z (2013) Comparison of the RNA interference effects triggered by dsRNA and siRNA in Tribolium castaneum. Pest management science, 69, 781–6.

Zhou L-T, Jia S, Wan P-J et al. (2013) RNA interference of a putative S-adenosyl-L-homocysteine hydrolase gene affects larval performance in Leptinotarsa decemlineata (Say). Journal of insect physiology, 59, 1049–56.

Petek M, Turnšek N, Buh Gašparič M et al. (2012) A complex of genes involved in adaptation of Leptinotarsa decemlineata larvae to induced potato defense. Archives of Insect Biochemistry and Physiology, 79, 153–81.

Bolognesi R, Ramaseshadri P, Anderson J et al. (2012) Characterizing the Mechanism of Action of Double-Stranded RNA Activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte). PLoS ONE, 7.

Rangasamy M, Siegfried BD (2012) Validation of RNA interference in western corn rootworm Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) adults. Pest Management Science, 68, 587–591.

Zhu F, Xu J, Palli R, Ferguson J, Palli SR (2011) Ingested RNA interference for managing the populations of the Colorado potato beetle, Leptinotarsa decemlineata. Pest management science, 67, 175–82.

Huvenne H, Smagghe G (2010) Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: a review. Journal of insect physiology, 56, 227–35.

Whyard S, Singh AD, Wong S (2009) Ingested double-stranded RNAs can act as species-specific insecticides. Insect biochemistry and molecular biology, 39, 824–32.

Baum JA, Bogaert T, Clinton W et al. (2007) Control of coleopteran insect pests through RNA interference. Nature biotechnology, 25, 1322–6.

Mao Y-B, Cai W-J, Wang J-W et al. (2007) Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol. Nature biotechnology, 25, 1307–13.