WP1: Development of advanced 3D in vitro spheroid models with enhanced liver-specific functions from hepatic cell lines

Task 1.1: To develop a 3D in vitro cell model under static conditions

Task 1.2: To develop a 3D in vitro cell model under dynamic conditions

WP2: 3D scaffolds with controlled architecture to generate 3D hepatic structure

Task 2.1: To prepare scaffolds from biocompatible biopolymers

Task 2.2: To develop the hepatic 3D in vitro cell model on scaffolds

WP3: Application of traditional methodologies of genotoxicology to 3D in vitro cell models

Task 3.1 To bring genotoxicology methodologies forward from 2D to 3D

Task 3.2: To study the cellular and molecular processes related to genotoxicity and cancer development in the newly developed 3D cell models

WP4: Validation of newly developed 3D in vitro models for genotoxicity assessment using a set of chemicals from the EURL ECVAM list

Task 4.1: To validate hepatic 3D in vitro cell models in the form of spheroids

Task 4.2: To validate hepatic 3D in vitro cell models developed on scaffolds

WP5: Analyses of gene and protein expression in the newly developed 3D in vitro cell models

Task 5.1: To identify the predictive biomarker genes involved in the metabolism and DNA damage response upon exposure to compounds from the EURL ECVAM list

Task 5.2: Protein expression of predictive biomarker genes for metabolism and genotoxic effects upon exposure to compounds from the EURL ECVAM list

WP6: Project management and dissemination activities

Task 6.1: Project and risk management activities

Task 6.2: Dissemination