May 2016 – RNAinVAL — Using RNA interference and Systems Biology approaches for validation of insecticide targets in Colorado potato beetle guts (dsRNA design and synthesis, validation of mode of dsRNA's action using RNA-seq gene expression profiling and NGS metagenomics/metatranscriptomics of beetle guts).

Monthly Archives: May 2016

Potato plants in the greenhouse

Posted on 24/05/2016 by Marko Petek • 0 comment

potted potato plants cv. Désirée to be used in CPB feeding experiments

Category: wet lab | Tags: greenhouse, potato

dsRNAs for CPB target genes synthesised

Posted on 23/05/2016 by Marko Petek • 0 comment

For each target gene a dsRNA was designed (the E-RNAi web service was used to optimise primer design) and synthesised in vitro by agroRNA. In addition, the positive and negative control dsRNA for the forthcoming feeding experiments were synthesised.

in vitro synthesised dsRNAs on a agarose gel

agarose gel of in vitro synthesised dsRNAs (50x diluted)

Category: research progress, wet lab | Tags: dsRNA design, dsRNA synthesis, electrophoresis, experimental controls