Project structure

The project is composed of four connected work packages (WPs):

WP1 is the controlling head of the project, checking every step and giving green light at each step, before going further. In WP2 the developed assays will be in-house validated and together with documentation transferred to WP3, which will check the methods and prepare everything for the validation in collaborative trial. WP4 will make statistical analysis of collaborative trial data.
 
Flow chart diagram below is illustrating the task dependencies, showing which tasks need to be done before others can be started. The green arrows show the documentation/material path through the work packages. The blue arrows show the data/results path through the work packages. Red arrows show the critical decision path through the work packages. Burst signs correspond to the chronology of the different activities:
     1. Transfer of documentation to WP2 and WP3,
     2. Results of in-house validation transferred to WP1 and analyzed,
     3. If continuation, transfer of documentation and material to WP3,
     4. Results of method verification and transferability study sent to WP1 and analyzed,
     5. If continuation, transfer of collaborative trial results to WP4,
     6. Results of collaborative trial transferred to WP1 and analyzed,
     7. Dissemination of documentation and results.
 

  

 

T1:

  • Preparation of documentation for WP2 and WP3. Selection of laboratories to participate to the transferability studies and the collaborative trials.

          Method in-house validation: the performance parameters to be tested, minimum requirements to reach these parameters           as well as result forms for reporting the performance of the methods. To WP2and WP3 partners.

          SOP template that considers the ISO requirements. To WP2 partners.

          Instructions regarding the preparation and the shipment of materials. To WP2 and WP3 partners

          

  • Selection of laboratories to participate to the transferability studies and the collaborative trials.

          Preparation of a questionnaire

          Definition of acceptance criteria

          List of potential participating laboratories

T2:

  • Complete in-house validation
  • Gather results and send to WP1

T3:

  • Produce/acquire material necessary for T2 and for WP3. Send non-extracted material and information about this material to WP3 partner.
  • Produce SOPs. Send to WP3partner.

T4:

  • Reception and registration of SOPs, material and related information sent by WP2 partners
  • Acquisition of the reagents for the following WP3 activities
  • DNA sample preparation

T5:

  • Characterization of the DNA samples
  • Experimental verification of some of method performances and transferability studies.
  • Data sent to WP1 partners.

T6:

  • Organisation of the collaborative trials (documentation, reagents and DNA samples shipment)
  • Gathering of the collaborative data
  • Data sent to FERA, WP1 partners and relevant method developer

T7:

  • Statistical analysis of collaborative trial data
  • Data sent to WP1 partners and relevant method developer

T8:

  • Assessment of data for each method in-house validation. Decision on continuation and data sent to WP3 partner and relevant method developer
  • Assessment of data for each method experimental verification and transferability study. Decision on continuation and data sent to WP3 partner and relevant method developer
  • Assessment of data for each method collaborative trial. Decision on the suitability of the method

T9:

  • Communication

Project GMOVal

The central aim of the project is to make available to the GMO laboratories community new validated real-time PCR screening methods for an improved GMO detection via the implementation of the matrix approach.